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Quote Originally Posted by The Bedlington Terrier View Post
What a phooking circus!







and sorry BT - forgot to post a Link.



Since PCR is based on DNA amplification, false-positive or -negative outcomes may easily occur. In particular, a single PCR cycle results in very large numbers of amplifiable molecules that can potentially contaminate subsequent amplifications of the same target sequence . In fact, a primary source of false-positive reactions has been identified as carryover of amplified product from previous reactions

C. pneumoniae is an obligate intracellular bacterium responsible for a variety of respiratory illnesses, including 10% of community-acquired pneumonias, bronchitis, pharyngitis, and sinusitis. Seroepidemiologically, 50 to 80% of the adult population has been shown to have prior exposure to this pathogen . Furthermore, recent studies have revealed that this bacterium may be involved in some chronic inflammatory diseases, such as asthma , arthritis , atherosclerosis, Alzheimer's disease , and MS . Since the culture of C. pneumoniae is difficult in most clinical laboratories, determination of this bacterium in clinical specimens has been widely performed using the PCR technique even though there is no standardized PCR method for detection of this organism. Therefore, PCR results with clinical specimens to detect this bacterium vary widely

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC119969/